Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.
نویسندگان
چکیده
We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
منابع مشابه
Evaluation of five real - time PCR assays for detection of Mycoplasma pneumoniae 7 8 Running title : Real - time PCR for detection of
25 Four commercial real-time PCR assays to detect M. pneumoniae were tested and the results 26 compared with an in-house approach. Despite differences of crossing threshold values up to 4, 27 assays were able to detect at least 20 cfu/5 μl (52 fg DNA/5 μl) of sample with best clinical 28 sensitivity of Diagenode kit. 29
متن کاملEvaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.
Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
متن کاملEvaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.
The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.
متن کاملCOMPARISON OF FOUR PCR TESTS FOR THE DETECTION OF MYCOPLASMA PNEUMONIAE
Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, det...
متن کاملIsolation and detection of Mycoplasma pneumoniae from cell culture by culture and PCR.
Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 46 9 شماره
صفحات -
تاریخ انتشار 2008